miR-486-5p enhances senescence of human mesenchymal stem cells by de-creasing telomerase activity / 中国病理生理杂志

AIM:To investigate the effects of microRNA-486-5p (miR-486-5p) on the senescence of human mesenchymal stem cells ( hMSCs).METHODS: The expression of miR-486-5p was determined by miRNA arrays and real-time PCR.By transfection of miR-486-5p mimic or inhibitor, up-regulation or down-regulation of miR-486-5p expres-sion in hMSCs was established.The effect of miR-486-5p and silence information regulator 1 (SIRT1) on hMSC telomerase activity and senescence were detected byβ-galactosidase staining.RESULTS:The expression of miR-486-5p was up-regu-lated in the old hMSCs compared with the young hMSCs.Up-regulation of miR-486-5p resulted in increasing senescence of hMSCs.Conversely, down-regulation of miR-486-5p resulted in decreasing cell senescence.The expression of SIRT1 and telomerase reverse transcriptase ( TERT) was down-regulated in the old hMSCs compared with the young hMSCs.Directly repression of SIRT1 expression inhibited the hMSC TERT protein expression and telomerase activity, but increased cell se-nescence.The regulation of miR-486-5p on hMSC senescence was attenuated by inhibiting the expression of miR-486-5p and SIRT1 together.CONCLUSION:miR-486-5p enhances senescence of hMSCs by decreasing the expression of SIRT1 and telomerase activity.

miR-708-5p accelerates migration of human mesenchymal stem cells by repressing TMEM88 expression / 中国病理生理杂志

AIM: To investigate the effect of microRNA-708-5p (miR-708-5p) on the migration of human mesenchymal stem cells (hMSCs).METHODS:The expression of miR-708-5p was determined by miRNA arrays and re-al-time PCR.By transfection of miR-708-5p mimic or inhibitor, the up-regulation or down-regulation of miR-708-5p ex-pression in hMSCs was evaluated .The cell scratch and Transwell tests were used to detect the migration capability of hM-SCs.The effects of transmembrane protein 88 (TMEM88), a miR-708-5p target gene, onβ-catenin expression and migra-tion of hMSCs were detected .RESULTS:The expression of miR-708-5p was down-regulated in the old hMSCs compared with the young hMSCs.Up-regulation of miR-708-5p resulted in increasing migration of hMSCs.Conversely, down-regula-tion of miR-708-5p resulted in decreasing cell migration .The expression of TMEM88 was up-regulated in the old hMSCs compared with the young hMSCs , while the expression of β-catenin was down-regulated.Directly repression of TMEM88 expression increased the β-catenin expression and migration of hMSCs .The regulation of miR-708-5p on hMSCs was atten-uated by inhibiting the expression of miR-708-5p and TMEM88 together.CONCLUSION:miR-708-5p increases β-cate-nin expression and Wnt/β-catenin activity by repressing TMEM 88, thus enhancing the migration of hMSCs .

MicroRNA-378*enhances apoptosis of human mesenchymal stem cells by repressing expression of CTGF / 中国病理生理杂志

[ ABSTRACT] AIM:To investigate the effects of microRNA-378*( miR-378*) on the survival and apoptosis of human mesenchymal stem cells ( hMSCs ) .METHODS: The expression of miR-378* was determined by microRNA arrays and quantitative real-time PCR ( qRT-PCR) .H2 O2 was used to induce hMSCs apoptosis.By transfection of miR-378*mimic or inhibitor, we up-regulated or down-regulated miR-378* expression in hMSCs.The effect of miR-378*and connective tissue growth factor ( CTGF) on hMSC survival and apoptosis were detected by MTT, LDH, caspase-3/7 and TUNEL assays.RESULTS:The expression of miR-378*was up-regulated in the old hMSCs compared with the young hMSCs.H2 O2 increased the expression of miR-378*, decreased the expression of CTGF.Up-regulation of miR-378*re-sulted in increasing apoptosis and decreasing survival of hMSCs.Conversely, down-regulation of miR-378*resulted in de-creasing cell apoptosis and increasing survival.The regulation of miR-378*on hMSC apoptosis and survival was attenuated by inhibiting the expression of miR-378* and CTGF together.Direct repression of CTGF expression inhibited the hMSC survival and increased apoptosis.CONCLUSION:miR-378*enhances apoptosis of hMSCs by repressing the expression of CTGF.

The studies on the properties of dendritic cells from human cord blood and malignant pleural effusions / 肿瘤研究与临床

Objective To investigate the culture and proliferation of dendritic cells from human cord blood and malignant pleural effusions and identify the phenotype and the function of them in vitro. Methods Existing DCs were obtained from cord blood and malignant pleural effusions. The DCs were cultured with IL-4, GM-CSF and TNF-?. The morphological properties of DCs were observed by invert optical microscope. The phenotypes of DCs were analysed by flow cytometry. The proliferative bioactivities of T cells activated by DCs were observed with mixed lymphocyte reaction (MLR). Results No remarkable differences were found in the morphology and cellular phenotype of the DC, derived from two different sources, but there was remarkable difference between their culture time. The DCs from two different sources could activate homologous lymphocytes to proliferate mostly. But the capacity to stimulate the proliferation of homologous lymphocytes had remarkable difference. Conclusion Mature DCs could be induced from cord blood and malignant pleural effusions.

STUDY ON EFFECT OF SPIRULINA PLATENSIS POLYSACCH-ARIDE ON NK CELLS FROM ACUTE LEUKEMIA PATIENT IN VITRO / 中国海洋药物

AIM:The Effects of Spirulina Platensis Polysaccharide (SPP)on peripheral NK cell from healthy persons and acute leukemia patients were studied.METHODS:The activities of NK cell on target cell K 562 was measured with MTT method.RESULTS:The results showed that SPP could argument the NK cell activity from varies peroid cases with acute leukemia.SPP had no activity on NK cell from normal person.Cytotoxicity did not present when the peripheral blood mononuclear cell were co incubated with SPP.CONCLUSION:These results suggested that SPP could be exploited and utilized as an approach of biological responsive modifier therapy in the treatment of acute leukemia.

Expression of DNA repair gene ERCC1 and its relationship with PAH-DNA adducts in lung cancer tissues / 中国病理生理杂志

AIM: To investigate the expression of nucleotide excision repair gene ERCC1 and its relationship with PAH (polycyclic aromatic hydrocarbons)-DNA adducts in lung cancer tissues. METHODS: ERCC1 mRNA expression and the PAH-induced DNA adducts were detected in 150 lung cancer tissues, 120 adjacent lung tissues without cancer cells, 40 benign lung lesions and 40 normal lung tissues. The effects of some exposure factors on the expression of ERCC1 gene and the connection between ERCC1 and PAH-DNA adduct was analyzed. RESULTS: Reduced expression levels of ERCC1 were observed in 46 of 150 (30.7%) lung cancer specimens and 1 of 40 (2.5%) normal lung tissues. Smoking may suppress the expression of ERCC1 gene. The level of PAH-DNA adduct was negatively correlated with the expression of ERCC1 gene, the Spearman coefficient was -0.648, P

The clinical efficacy of capecitabine administrated continuously for three weeks in treating old patients with advanced digestive tract cancer / 中国癌症杂志

0.05) between the control group and the conventional group.Incidences of hand-foot syndrome, nausea, vomiting, stomatitis and diarrhea were significantly lower in the control group in comparison to the conventional group(P

Expression and clinical significance of nm23-H_1 and nm23-H_2 in human breast cancer / 中国癌症杂志

Purpose:To investigate the expression and clinical significance of nm23 H 1 and nm23 H 2 in human breast cancer. Methods:A semi quantitative reverse transcription polymerase chain reaction was performed to detect the expression of nm23 H 1 and nm23 H 2 mRNA in thirty human breast cancer specimens. To evaluate the relationship between nm23 gene and breast cancer metastasis t test, F test and multivariate analysis were used.Results:The nm23 H 1 mRNA level in primary breast cancer tissue with lymph node positive was lower than that with lymph node negative. The nm23 H 1 mRNA level in breast cancer with Ⅰ,Ⅱstage was higher than that with Ⅲ stage. Lymph node metastasis was the only parameter that was significantly correlated with the nm23 H 1 expression. There was no significant relation between nm23 H 2 and tumor size, lymph node status, TNM stage, hormone receptor and menopausal status.Conclusions:nm23 H 1 mRNA show a significant inverse correlation with lymph node metastasis. nm23 H 1 appears to play a more important role than nm23 H 2 in breast cancer. nm23 H 1 mRNA expression may be a predictor of lymph node metastasis.